Thymosin Beta-4 Fragment (17-23) 5 mg × 10 vials — Research-Grade Actin-Sequestering Peptide Fragment

Document ID: HKPW-tb-500-5-mg-thymosin-beta-research-pepti-v1.0 | Reviewed by: HKPEPTIDE WORLDWIDE Research Team | Last Updated: 2026-08-08

Product Identity & Specifications

Parameter	Specification
Product Name	Thymosin Beta-4 Fragment (17-23)
Dosage	5 mg × 10 vials
CAS Number	77591-33-4
Molecular Formula	C₃₈H₆₈N₁₀O₁₄
Molecular Weight	889 Da
Sequence	Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH
Purity	≥99% HPLC
Appearance	White to off-white lyophilized powder
Solubility	Soluble in sterile water or bacteriostatic water
Storage	2–8°C, desiccated, protected from light
Classification	Actin-Sequestering Peptide Fragment

Research Background

TB-500 is the synthetic N-terminal fragment (residues 17-23) of Thymosin Beta-4. This 7-amino acid peptide retains the complete actin-binding domain responsible for TB4’s effects on cell migration and angiogenesis. By sequestering G-actin monomers (1:1 binding, Kd ~0.7 μM), TB-500 maintains the cellular actin monomer pool essential for rapid cytoskeletal reorganization during wound healing and tissue regeneration.

The 5 mg × 10 vials configuration provides an optimal quantity for [specific protocol type] research designs. Each vial is manufactured under strict quality control protocols with full analytical documentation including HPLC purity verification and mass spectrometry identity confirmation.

Molecular Mechanisms

G-Actin Sequestration: 1:1 binding to monomeric actin → maintains polymerization-ready actin pool. Essential for rapid cytoskeletal reorganization during cell migration, lamellipodia formation, and cytokinesis.

Cell Migration: Actin dynamics → lamellipodia and filopodia formation → directional keratinocyte and endothelial cell migration into wound beds.

Anti-Inflammatory: Suppresses NF-κB nuclear translocation → reduced TNF-α, IL-6. Promotes M2 macrophage polarization → inflammation resolution.

Anti-Apoptotic: Akt/Bad phosphorylation → mitochondrial stabilization → reduced cytochrome c release and caspase-3 activation during ischemic stress.

Research Applications

Wound Healing & Tissue Regeneration
Angiogenesis Research
Cardiac Protection Models
Inflammation Resolution
Cell Migration & Cytoskeletal Dynamics

Comparative Analysis

Compared to BPC-157: TB-500 drives cell migration via actin sequestration; BPC-157 drives angiogenesis via VEGF upregulation. Combined: synergistic tissue repair. Compared to full Thymosin Beta-4 (43 aa): TB-500 is the minimal active actin-binding domain — simpler, more stable, cost-effective for actin-focused research.

Quality Control & Analytical Specifications

Parameter	Method	Specification
Purity	HPLC at 214 nm, C18 column	≥99% HPLC
Identity (MW)	ESI-MS	889 Da
Appearance	Visual inspection	White to off-white powder
Peptide Content	Amino acid analysis	≥80% net peptide
Residual Moisture	Karl Fischer titration	≤3%
Endotoxin	LAL assay	≤0.5 EU/mg
Sterility	Membrane filtration	Sterile-filtered

Available Configurations

Configuration	Content	Best For
Standard Kit	5 mg × 10 vials × 10 vials	Evaluation; protocol validation
Bulk Kit	Custom dosage × 10 vials	Chronic studies; repeat orders
OEM/Private Label	Custom configurations	Branded product development

Frequently Asked Questions

Q: How does TB-500 differ from Thymosin Beta-4?

TB-500 is the 7-aa actin-binding domain (LKKTETQ). Full TB4 is 43 aa including additional regulatory regions. TB-500 is preferred for actin-specific studies; TB4 for full biological context.

Q: What is G-actin sequestration?

TB-500 binds monomeric G-actin 1:1, preventing spontaneous polymerization while maintaining the actin pool ready for rapid filament assembly when needed. This actin-buffering is critical for cell motility.

Q: How should TB-500 be stored?

Lyophilized: 2-8°C, desiccated. Reconstitute with sterile PBS. Aliquot, store at -20°C. Solution stability: ~14 days at 2-8°C.

Q: What documentation is provided?

Batch-specific COA: HPLC purity, ESI-MS identity, amino acid analysis, Karl Fischer moisture. Full analytical package on request.

Tiered Wholesale Pricing

Volume	Discount	For
1 kit	List price	Evaluation; competitive analysis
5+ kits	10% off	Initial portfolio expansion
20+ kits	20% off	Mid-size distributors
50+ kits	30% off	Regional distributors

Contact our team for custom volume pricing on 100+ kit orders.

References & Further Reading

Scientific literature available upon request for qualified research institutions
Batch-specific Certificate of Analysis (COA) included with every order
Full HPLC chromatogram and MS spectrum available on request
Technical dossier provided with bulk wholesale orders

Resource	Description
Healing Regenerative Peptides Hub	Complete research overview & methodology hub
Bpc 157 Tb 500 10 Mg Research Peptide Co	Related research peptide product
Bpc 157 Tb 500 20 Mg Research Peptide Us	Related research peptide product
The Growing Interest In Peptides For Muscle Research	Latest research insights & methodology

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Compliance Statement

FOR LABORATORY RESEARCH USE ONLY. This product is intended exclusively for in vitro laboratory research and scientific investigation. Not for human or veterinary diagnostic, therapeutic, or prophylactic use. Not a dietary supplement, pharmaceutical ingredient, or approved drug substance. All researchers must comply with applicable institutional, local, and national regulations governing research chemical use.

TB-500 5 mg