Nicotinamide Adenine Dinucleotide (NAD+) 500 mg — Research-Grade Essential Cellular Coenzyme

Document ID: HKPW-nad-e2-81-ba-500-mg-cellular-metabolism—v1.0 | Reviewed: HKPEPTIDE WORLDWIDE Research Team | Updated: 2026-08-08

Product Identity & Specifications

Parameter	Specification
Product	Nicotinamide Adenine Dinucleotide (NAD+)
Dosage	500 mg
CAS	53-84-9
Formula	C21H27N7O14P2
MW	~663.4 Da
Sequence	beta-Nicotinamide Adenine Dinucleotide, oxidized form
Purity	≥99% HPLC
Appearance	White to off-white lyophilized powder
Solubility	Sterile water or bacteriostatic water
Storage	2–8°C, desiccated, protected from light
Class	Essential Cellular Coenzyme

Research Background

Nicotinamide Adenine Dinucleotide (NAD+) is a fundamental coenzyme present in every living cell, essential for redox reactions in energy metabolism and as a substrate for NAD+-consuming enzymes including sirtuins (SIRT1-7), PARPs, and CD38. Cellular NAD+ levels decline with age across multiple tissues, implicated in mitochondrial dysfunction, metabolic dysregulation, and cellular senescence.

The 500 mg configuration is manufactured under strict quality control with HPLC, MS, and AAA verification. Batch-specific COA included. Bulk and OEM configurations available.

Molecular Mechanisms

Redox Cofactor (NAD+/NADH)

Central electron carrier in cellular respiration: glycolysis, TCA cycle, and oxidative phosphorylation. The NAD+/NADH ratio is a key metabolic sensor regulating SIRT1 activity.

Sirtuin Activation

NAD+ is the obligate substrate for sirtuin-catalyzed deacetylation. SIRT1 deacetylates PGC-1alpha, FOXO, p53, promoting mitochondrial biogenesis and stress resistance.

PARP Substrate

PARP1 uses NAD+ to synthesize poly(ADP-ribose) chains during DNA damage repair. Excessive PARP activation can deplete cellular NAD+.

CD38 NADase

CD38 is a major NAD+-consuming enzyme on immune cells. CD38 activity increases with age accelerating NAD+ decline.

Research Applications

Cellular Bioenergetics
Sirtuin Research
NAD+/NADH Redox Biology
Aging and Longevity
PARP and DNA Repair

Comparative Analysis

Compared to NMN and NR: NAD+ is the direct coenzyme with no biosynthetic conversion required. NMN and NR are precursors requiring cellular uptake and enzymatic conversion to NAD+. NAD+ is preferred for in vitro studies requiring direct coenzyme availability.

Quality Specifications

Parameter	Method	Specification
Purity	HPLC 214nm C18	≥99.0%
Identity	ESI-MS	~663.4 Da
Appearance	Visual	White to off-white powder
Peptide Content	AAA	≥80% net peptide
Moisture	Karl Fischer	≤3%
Endotoxin	LAL	≤0.5 EU/mg

Available Configurations

Configuration	Content	Best For
Standard Kit	500 mg × 10 vials	Evaluation
Bulk Kit	Custom × 10 vials	Chronic studies
OEM	Custom	Branded products

FAQ

Q: NAD+ vs NMN vs NR?

NAD+ is the active coenzyme. NMN and NR are biosynthetic precursors. NAD+ = direct coenzyme. NMN/NR = precursors requiring enzymatic conversion.

Q: Why does NAD+ decline with age?

Multiple mechanisms: increased CD38 NADase, DNA damage-induced PARP activation consuming NAD+, decreased NAMPT expression, and mitochondrial dysfunction.

Wholesale Pricing

Volume	Discount	For
1 kit	List price	Evaluation; competitive analysis
5+ kits	10% off	Initial portfolio expansion
20+ kits	20% off	Mid-size distributors
50+ kits	30% off	Regional distributors

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Compliance

FOR LABORATORY RESEARCH USE ONLY. Not for human or veterinary use. Not a dietary supplement or pharmaceutical.

NAD+ 500 mg